INTACT RECOMBINANT CELLS OF THE YEAST HANSENULA POLYMORPHA, OVER-PRODUCING FORMALDEHYDE DEHYDROGENASE, AS THE SENSITIVE BIOELEMENTS FOR AMPEROMETRIC ASSAY OF FORMALDEHYDE
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چکیده
منابع مشابه
Bioconversion of airborne formaldehyde by immobilized formaldehyde dehydrogenase from the recombinant methylotrophic yeast Hansenula polymorpha
Formaldehyde (FA) is a very toxic pollutant. FA in indoor air has a negative effect on human health and should be removed by ventilation or by conversion to non-toxic products. The formaldehyde dehydrogenase (FdDH), a NADand glutathione-dependent enzyme from recombinant methylotrophic yeast Hansenula polymorpha, was tested for its ability to oxidize airborne FA. A continuous fluidized bed biore...
متن کاملMolecular characterization of the Hansenula polymorpha FLDI gene encoding formaldehyde dehydrogenase
Richard J. S. Baerends,{, Grietje J. Sulter, Thomas W. Jeffries, James M. Cregg and Marten Veenhuis* 1 Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands 2 Institute for Microbial and Biochemical Technology, Forest Products Laboratory, US Department of Agriculture, One Gifford Pinchot Drive, ...
متن کاملMolecular characterization of the Hansenula polymorpha FLD1 gene encoding formaldehyde dehydrogenase.
Glutathione-dependent formaldehyde dehydrogenase (FLD) is a key enzyme required forthe catabolism of methanol as a carbon source and certain primary amines, such as methylamine as nitrogen sources in methylotrophic yeasts. Here we describe the molecular characterization of the FLD1 gene from the yeast Hansenula polymorpha. Unlike the recently described Pichia pastoris homologue, the H. polymorp...
متن کاملFormaldehyde dehydrogenase from bakers' yeast.
In the course of studies of formaldehyde metabolism in yeast (I), an enzyme was found that catalyzed the oxidation of formaldehyde in the presence of glutathione and diphosphopyridine nucleotide. A similar enzyme was discovered independently by Strittmatter and Ball in liver (2). This paper describes the partial purification and some of the properties of yeast formaldehyde dehydrogenase. Some e...
متن کاملExpression of recombinant staphylokinase in the methylotrophic yeast Hansenula polymorpha
BACKGROUND Currently, the two most commonly used fibrinolytic agents in thrombolytic therapy are recombinant tissue plasminogen activator (rt-PA) and streptokinase (SK). Whereas SK has the advantage of substantially lower costs when compared to other agents, it is less effective than either rt-PA or related variants, has significant allergenic potential, lacks fibrin selectivity and causes tran...
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ژورنال
عنوان ژورنال: Sensor Electronics and Microsystem Technologies
سال: 2008
ISSN: 2415-3508,1815-7459
DOI: 10.18524/1815-7459.2008.2.114427